авг 27, 2017
New Material Enables High-Quality Bioimaging
A nanosheet made of organic polymers has been developed to prevent the drying and deforming of biological samples, thus enabling high-quality imaging under microscopes.
Scientists from Hokkaido University and Tokai University in Japan have developed a nanosheet made of a commercially available, fluorine-containing polymer known as CYTOP® and investigated its effectiveness in producing clear images when wrapped around biological samples.
The researchers successfully produced a nanosheet of CYTOP® by spreading its solution on a layer of poly-vinyl-alcohol (PVA). The nano-thin layer of CYTOP® was easily detached from the PVA layer by soaking them in water. The thickness of the nanosheet could be adjusted by changing the concentration of its solution. The nanosheet retained CYTOP®'s high water repellent properties which should help retain a sample's water content when used as wrapper.
The team then tested the nanosheet's wrapping properties by using alginate hydrogel to simulate biological samples such as tissue. They found that the gel retained 60% of its water content after 24 hours when wrapped whereas the unwrapped gel became totally dehydrated after about 10 hours. Experimenting with various thicknesses, they found that a 133-nm-thick sheet provides sufficient water retention and surface adhesion which is necessary for fixing samples to the cover slip.
They next applied an actual biological sample: 1-mm thick brain slices from mice. Without a CYTOP® wrap, evaporation of the embedded water caused local, non-uniform sample shrinkage, leading to blurred images. By wrapping the brain slices in a CYTOP® nanosheet, however, images with a high spatial resolution could be obtained from scanning a large area (more than 750 µm x 750 µm) over a long time (about 2 hours). Similar results were obtained even when the sample was embedded in agarose gel — a common technique used for mounting biological tissues that could disturb the clarity of taransparentized samples — without disturbing the clarity.
"The wrapping technique is still at an early stage, but it establishes and verifies the superiority of nanosheet wrapping for tissue imaging," say the researchers of the paper published in Advanced Materials.
This article has been republished from materials provided by Hokkaido University. Note: material may have been edited for length and content. For further information, please contact the cited source.
Zhang H. et al., 'Fluoropolymer Nanosheet as a Wrapping Mount for High-Quality Tissue Imaging'. Advanced Materials, (August 2017). DOI: 10.1002/adma.201703139